Time-Resolved Fluorescence Wiki

measuring_quantum_yield

Anonymous (anonymous@undisclosed.example.com) — 2017-07-12T13:16:44+00:00

2017/07/12 15:13		current
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	{{ youtube>JLiDnlS5BS8?large }}		{{ youtube>JLiDnlS5BS8?large }}

-	===== Setp-by-Step =====	+	===== Step-by-Step =====
-	* Insert the integrating sphere with caution in order not to hit any optical elements in the FluoTime 300. The integrating sphere assembly has an indexed lid to ensure proper orientation.	+	* Mount the integrating sphere carefully. Make sure you do not hit any optical elements inside the FluoTime 300. The integrating sphere assembly has an indexed lid to ensure proper orientation.

	{{ :howto:2013-12-19_at_14-30-03.jpg?600 \|}}		{{ :howto:2013-12-19_at_14-30-03.jpg?600 \|}}

2ffcs

Anonymous (anonymous@undisclosed.example.com) — 2015-11-04T09:41:00+00:00

2014/04/09 22:02		current
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	Correlation analysis that is applied to the fluctuations of the fluorescence intensity. The cross-correlation between the occurrence of events in two different detection volumes is used to introduce an absolute diffusion length into the analysis.		Correlation analysis that is applied to the fluctuations of the fluorescence intensity. The cross-correlation between the occurrence of events in two different detection volumes is used to introduce an absolute diffusion length into the analysis.
		+
		+	see [[glossary:FCS]] for a introduction on FCS.

	===== Experimental Setup =====		===== Experimental Setup =====

align_beam_backreflection

Anonymous (anonymous@undisclosed.example.com) — 2022-08-29T11:17:12+00:00

2022/08/29 13:15		current
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	Adding a drop of water on top of the coverslip reduces the back-scattering intensity by an order of magnitude or so, but the basic shape remains the same. Even if one removes everything extra from the beam path, these fringes remain there, they are universal. Note that all those images above are slightly defocused.		Adding a drop of water on top of the coverslip reduces the back-scattering intensity by an order of magnitude or so, but the basic shape remains the same. Even if one removes everything extra from the beam path, these fringes remain there, they are universal. Note that all those images above are slightly defocused.

-	Such images are used for the daily and fundamental alignment of the MicroTime 200. The detailed procedure is described in the user manual of MicroTime 200. Although the MicroTime is extremely robust we suggest to spend 5-10 minutes every day for verifying the quality of the alignment by inspecting the back-reflection pattern. Have a look how this is used also by watching the tutorial [[howto:exchange_dichroic_mt200\|How to exchange the main dichroic of the MicroTime 200.]]	+	Such images are used for daily and fundamental alignment of the MicroTime 200. A detailed procedure is described in the user manual of MicroTime 200. Although MicroTime is extremely robust, we suggest to spend 5-10 minutes every day for verifying the quality of the alignment by inspecting the back-reflection pattern. Have a look how this is used also by watching the tutorial [[howto:exchange_dichroic_mt200\|How to exchange the main dichroic of the MicroTime 200.]]

	The paper by [[ http://rspa.royalsocietypublishing.org/content/253/1274/358\|B. Richards and E. Wolf: Electromagnetic diffraction in optical systems, II. Structure of the image field in an aplanatic system]] shows such 4 lobed patterns for polarized light.		The paper by [[ http://rspa.royalsocietypublishing.org/content/253/1274/358\|B. Richards and E. Wolf: Electromagnetic diffraction in optical systems, II. Structure of the image field in an aplanatic system]] shows such 4 lobed patterns for polarized light.

	Similar figures can be found in this [[https://books.google.cz/books?id=kURdAAAAQBAJ\|classic textbook]].		Similar figures can be found in this [[https://books.google.cz/books?id=kURdAAAAQBAJ\|classic textbook]].

diamond_nv_centers

Anonymous (anonymous@undisclosed.example.com) — 2018-04-19T12:11:55+00:00

2018/04/19 14:11		current
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	see also [[wp>Nitrogen-vacancy center]]		see also [[wp>Nitrogen-vacancy center]]

-	{{ howto:model_nv-centers.png\|Figure1: Model of the nitrogene vacancy in the diamond lattice}}	+	[{{ howto:model_nv-centers.png\|Figure1: Model of the nitrogene vacancy in the diamond lattice}}]

	==== Excitation ====		==== Excitation ====

how_to_measure_the_instrument_response_function_irf

Anonymous (anonymous@undisclosed.example.com) — 2023-09-07T22:55:03+00:00

2023/09/08 00:54		current
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	===== Using samples with ultrafast decay =====		===== Using samples with ultrafast decay =====

-	Some detectors (particularly SPADs) have wavelength dependent timing response. In this case an IRF recorded at the excitation wavelength may not be useful for precise reconvolution. The solution is to acquire the IRF at the fluorescence wavelength, or at least spectrally closer to the fluorescence emission.	+	Some detectors (particularly MPD SPADs) have a wavelength dependent timing response. In this case an IRF recorded at the excitation wavelength may not be useful for precise reconvolution. The solution is to acquire the IRF at the fluorescence wavelength, or at least spectrally closer to the fluorescence emission wavelength.

	==== General recipe ====		==== General recipe ====

how_to_work_with_the_instrument_response_function_irf

Anonymous (anonymous@undisclosed.example.com) — 2023-02-17T12:41:49+00:00

2021/03/23 11:49		current
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-	{{tag>howto IRF tutorial analysis MT LSM_upgrade}}	+	{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}

intensity_time_trace_analysis

Anonymous (anonymous@undisclosed.example.com) — 2014-06-19T08:12:32+00:00

2014/06/19 10:12		current
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	{{tag> tutorial howto time-trace analysis SymPhoTime}}		{{tag> tutorial howto time-trace analysis SymPhoTime}}

-	~~TOC~~	+	~~NOTOC~~

	======Intensity Time Trace Analysis======		======Intensity Time Trace Analysis======

select_the_correct_pinhole_size

Anonymous (anonymous@undisclosed.example.com) — 2015-09-11T15:24:14+00:00

2015/09/11 17:22		current
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	====== How-to select the correct pinhole size ======		====== How-to select the correct pinhole size ======

-	The pinhole size should be slightly bigger than the image of the [[https://en.wikipedia.org/wiki/Airy_disk\|Airy Disc]] at the position of the pinhole:	+	The pinhole size should be slightly bigger than the image of the [[wp>Airy Disc]] at the position of the pinhole:

	$$d_{Airy} = (1.22 * lambda / N.A.) * M$$		$$d_{Airy} = (1.22 * lambda / N.A.) * M$$

using_the_antibunching_script

Anonymous (anonymous@undisclosed.example.com) — 2014-06-19T07:40:32+00:00

2014/06/19 09:36		current
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	{{tag> antibunching howto tutorial correlation analysis SymPhoTime}}		{{tag> antibunching howto tutorial correlation analysis SymPhoTime}}
-	~~TOC~~	+	~~NOTOC~~

	====== Antibunching Analysis ======		====== Antibunching Analysis ======
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	This tutorial shows step-by-step, how to calculate an antibunching curve from a fluorescence time trace of a single emitter, in this example a nanodiamond NV center, excited with a pulsed 530 nm laser.		This tutorial shows step-by-step, how to calculate an antibunching curve from a fluorescence time trace of a single emitter, in this example a nanodiamond NV center, excited with a pulsed 530 nm laser.
		+
		+	{{ youtube>bEyhuWnmFC4?large }}

	===== Background Information =====		===== Background Information =====
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	* Here it is impossible to determine the number of emitters. In this measurement the luminescence stems from several emitters.		* Here it is impossible to determine the number of emitters. In this measurement the luminescence stems from several emitters.
-

burst_duration_and_fcs_diffusion_time

Anonymous (anonymous@undisclosed.example.com) — 2026-01-12T14:58:02+00:00

2026/01/12 15:56		current
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	Gopich, I. V.; Szabo, A. Journal of Chemical Physics 2005, 122, 014707.		Gopich, I. V.; Szabo, A. Journal of Chemical Physics 2005, 122, 014707.

-	Ruttinger, S. Dissertation, 2006.	+	Ruettinger, S. Dissertation, 2006.

-	Dorr, D. Dissertation, 2014.	+	Doerr, D. Dissertation, 2014.