Time-Resolved Fluorescence Wiki
https://www.tcspc.de/
2026-04-23T20:05:48+00:00Time-Resolved Fluorescence Wiki
https://www.tcspc.de/
https://www.tcspc.de/lib/exe/fetch.php/logo.pngtext/html2025-02-14T15:49:47+00:00Anonymous (anonymous@undisclosed.example.com)how_can_i_analyze_my_data_using_non-picoquant_software
https://www.tcspc.de/doku.php/faq:how_can_i_analyze_my_data_using_non-picoquant_software?rev=1739548187&do=diff
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<tr><td class="diff-lineheader"> </td><td class="diff-context">====== How can I analyze my data using non-PicoQuant software? ======</td><td class="diff-lineheader"> </td><td class="diff-context">====== How can I analyze my data using non-PicoQuant software? ======</td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context"></td><td class="diff-lineheader"> </td><td class="diff-context"></td></tr>
<tr><td class="diff-lineheader">-</td><td class="diff-deletedline">{{tag> faq file_format ptu open_source analysis}}</td><td class="diff-lineheader">+</td><td class="diff-addedline">{{tag> faq file_format ptu open_source analysis <strong class="diff-mark">software</strong>}}</td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context"></td><td class="diff-lineheader"> </td><td class="diff-context"></td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context">The PicoQuant raw-data format is freely accessible. PicoQuant has published some demo scripts for reading the different TCSPC/TTTR data formats on Github:</td><td class="diff-lineheader"> </td><td class="diff-context">The PicoQuant raw-data format is freely accessible. PicoQuant has published some demo scripts for reading the different TCSPC/TTTR data formats on Github:</td></tr>
</table>text/html2025-03-11T08:19:29+00:00Anonymous (anonymous@undisclosed.example.com)novaflim_calculated_irf
https://www.tcspc.de/doku.php/faq:novaflim_calculated_irf?rev=1741681169&do=diff
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<tr><td class="diff-lineheader"> </td><td class="diff-context">====== NovaFLIM Calculated IRF ======</td><td class="diff-lineheader"> </td><td class="diff-context">====== NovaFLIM Calculated IRF ======</td></tr>
<tr><td colspan="2"> </td><td class="diff-lineheader">+</td><td class="diff-addedline">{{tag> faq software novaflim IRF}}</td></tr>
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<tr><td class="diff-lineheader"> </td><td class="diff-context">In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit. </td><td class="diff-lineheader"> </td><td class="diff-context">In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit. </td></tr>
</table>text/html2025-02-14T09:43:52+00:00Anonymous (anonymous@undisclosed.example.com)start
https://www.tcspc.de/doku.php/faq:start?rev=1739526232&do=diff
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<tr><td class="diff-lineheader"> </td><td class="diff-context">**In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).**</td><td class="diff-lineheader"> </td><td class="diff-context">**In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).**</td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context"></td><td class="diff-lineheader"> </td><td class="diff-context"></td></tr>
<tr><td class="diff-lineheader">-</td><td class="diff-deletedline">If you are using either a **proper** (see below) 50/50 <strong class="diff-mark">cube </strong>beamsplitter to split the signal between two detectors or a 100% mirror to direct the signal to one detector and then the other, but still observe very different count rates on the two detectors, this discrepancy could be due to the following reasons:</td><td class="diff-lineheader">+</td><td class="diff-addedline">If you are using either a **proper** (see below) 50/50 beamsplitter to split the signal between two detectors or a 100% mirror to direct the signal to one detector and then the other, but still observe very different count rates on the two detectors, this discrepancy could be due to the following reasons:</td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context"></td><td class="diff-lineheader"> </td><td class="diff-context"></td></tr>
<tr><td class="diff-lineheader"> </td><td class="diff-context">1. Misalignment of the Lens in Front of the SPAD:</td><td class="diff-lineheader"> </td><td class="diff-context">1. Misalignment of the Lens in Front of the SPAD:</td></tr>
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